How does a hydroxyproline assay work?
In the Hydroxyproline Assay Kit, hydroxyproline concentration is determined by the reaction of oxidized hydroxyproline with 4-(dimethylamino)benzaldehyde (DMAB), which results in a colorimetric (560 nm) product, proportional to the hydroxyproline present.
What does hydroxyproline assay measure?
Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present. The QuickZyme Hydroxyproline assay is a modification of the method described by Prockop and Udenfriend (Anal. Biochem.,1960, 1: 228- 239). The assay measures the total amount of hydroxyproline present in the sample.
What is required for hydroxyproline formation?
Proline hydroxylation requires ascorbic acid (vitamin C). The most obvious, first effects (gingival and hair problems) of absence of ascorbic acid in humans come from the resulting defect in hydroxylation of proline residues of collagen, with reduced stability of the collagen molecule, causing scurvy.
How do you convert hydroxyproline to collagen?
D = mg hydroxyproline / total lung; Collagen conversion factor = 13.5; × = mg collagen / total lung. Neuman et al. reported that approximately 13.5% of collagen is composed of hydroxyproline , thus this value was used as the conversion factor.
How do you measure collagen content?
The most widely used collagen assay is based on hydrolysis of collagen to free amino acids, followed by measuring collagen specific hydroxyproline, with either HPLC or a colorimetric method. However, these assays are laborious, time consuming and require special equipment.
What foods contain hydroxyproline?
Hydroxyproline is found in the connective tissue and collagen components in animal meat, offal, organ meats, bone broth, collagen foods, and supplements. Finding it in plant sources is hard, often not yielding adequate amounts, but of the highest content includes Alfalfa Sprouts.
Is hydroxyproline good for skin?
Hydroxyproline has the potential to decrease fine lines and wrinkles when used topically and also helps to increase penetration into the skin of other anti-aging skin care ingredients. In addition, hydroxyproline binds water, making it an effective moisturizer and skin conditioner.
What is a hydroxyproline assay kit?
BioAssay Systems’ Hydroxyproline assay kit delivers a safe, simple, and sensitive means to quantify hydroxyproline in samples. In the first step of this procedure, hydroxyproline in the sample is oxidized to a pyrrole ring.
How do you quantify hydroxyproline?
BioAssay Systems’ Hydroxyproline assay kit delivers a safe, simple, and sensitive means to quantify hydroxyproline in samples. In the first step of this procedure, hydroxyproline in the sample is oxidized to a pyrrole ring. This compound then reacts with a dye reagent to yield a pink product that can be measured at 560 nm.
Can hydroxyproline assay be used to measure collagen content in tissue homogenates?
A critical study of the different steps involved in previous procedure for hydroxyproline assay allows the direct measurement of collagen content in tissue homogenates without losing the advantages of the method.
How much does a hydroxyproline test cost?
Price: $429 Qty: For orders of 10 or more kits, please call +1-510-782-9988×1 or email us for best pricing and/or bulk order. Direct Assays: Hydroxyproline and collagen in biologic and cosmetic samples. Safe. Our hydroxyproline assay uses an improved perchlorate-free chemistry. Sensitive and accurate. Uses 20 µL sample.