Does horse serum need to be heat inactivated?
Horse serum is available in both a heat inactivated and non-heat inactivated format. HI serum is heated for 30 minutes at 56°C while mixing to inactivate the complement.
Can you over heat inactivate serum?
Notes: Heating the serum for longer than 30 minutes or higher than 56°C will have an adverse effect on the efficacy of the serum and will most likely cause an increase in the amount of cryo-precipitate that might form.
What is the purpose of heat inactivation of serum?
Abstract. Heating serum at 56 degrees is used to inactivate complement in several immunological assays. During heating, both heat-labile and heat-stable anticomplementary activity (ACA) develop. While heat-labile ACA can be completely inactivated, heat-stable ACA increases progressively with continued heating.
How do you heat inactivate sera?
If the user resolves to use heat inactivation, Seraglob recommends the following procedure: Perform heat inactivation at 56 °C for a period of 30 minutes by heating the laboratory-scale serum bottles in a water bath. During this process, stir the serum and shake it at least from time to time, while avoiding foaming.
What is the difference between fetal bovine serum and horse serum?
Fetal calf serum and Fetal Bovine serum are the same,but horse serum is different in growth factors. Conventionally, Fetal calf serum ismostly used for growing cells and depending on the type of cell and your protocol that will determine the FCS concentration.
What is donor horse serum?
Donor Horse Serum (DHS) is derived from healthy, live animals greater than 12 months of age. The donor animals receive regular veterinary inspection and care, and are kept in carefully managed and strictly segregated herds.
Can you heat inactivate FBS for too long?
so, I agree with previous comment, inactivate serum for longer time can reduce the nutritional components of FBS. *cells are stressed. 2 hours at 56 °C could destroy the proteins of FBS that are necessary to support the cell growth ( they are cooked).
How long is FBS stable at 37 degrees?
You should be able to store the medium at physiological relevant temperature (37 °C) for 9 days.
What is meant by serum inactivation?
in·ac·ti·vat·ed se·rum. serum that has been heated to 56°C for 30 minutes to destroy the lytic activity of complement.
What is heat inactivation?
Heat inactivation is a convenient method for stopping a restriction endonuclease reaction. Incubation at 65°C for 20 minutes inactivates the majority of restriction endonucleases that have an optimal incubation temperature of 37°C.
Why the heat inactivation of fetal calf serum FBS is done?
A common treatment of FBS is heat-inactivation, where FBS is heated at 56°C for 30 minutes in a water bath with occasional shaking. The purpose is to inactivate whatever components of the complement system are present in the FBS [24], and other potential unknown inhibitors of cell growth.
How do you compare results between reference and heat inactivated serum?
Results are compared to parallel results obtained using control growth medium containing a previously characterized reference serum. Horse serum is available in both a heat inactivated and non-heat inactivated format. HI serum is heated for 30 minutes at 56°C while mixing to inactivate the complement.
What are the common uses of horse serum?
Common Uses 1 10% horse serum could be used to support an in vitro antibody response to SRBC in place of FBS 1 2 Used in the production of C2 heterokaryons 2 3 Primary Neuronal Culture
What is GIBCO horse serum?
Horse Serum Gibco® horse serum is donor-herd collected, processed, and manufactured in New Zealand. Each lot of horse serum is tested for its ability to support the growth of Sp2/O-Ag14 (murine myeloma) cells in control medium containing the test lot of serum.
How long should Hihi serum be heated for?
HI serum is heated for 30 minutes at 56°C while mixing to inactivate the complement. Miller Levy, E., The ability of horse serum to support…, Journal of Immunological Methods Volume 36, Issue 2, 27 August 1980, Pages 181-183