What is rt2 Profiler PCR Array?
The RT² Profiler PCR Array is a 96-/384-well plate or 100-well disc that contains gene-specific Primer Assays for a thoroughly researched set of relevant, pathway- or disease-focused genes. It simultaneously profiles the expression of 84 pathway-specific genes, and five housekeeping genes.
What is a PCR array?
PCR arrays are dedicated to gene expression analysis by quantitative PCR. They are sets of primers, on the same support (384-well microplate), selected according to a theme or a given biological process.
What diseases can microarray detect?
A microarray is the recommended first line genetic test for developmental delay (DD), intellectual disability (ID) and autism spectrum disorders (ASD)*. CMA however, does not identify fragile X syndrome (FXS), a common cause of intellectual disability.
How can a microarray be used to diagnose a disease?
Diagnostic DNA microarrays have been used for genotyping and determination of disease-relevant genes or agents causing diseases, mutation analysis, screening of single nucleotide polymorphisms (SNPs), detection of chromosome abnormalities, and global determination of posttranslational modification.
What is an RT 2 profiler PCR array?
RT 2 Profiler PCR Arrays are available in 96-well plate, 384-well plate, and 100-well disc formats, and are used to monitor the expression of 84 or 370 genes related to a disease state or pathway, plus 5 housekeeping genes. Each RT 2 Profiler PCR Array also includes control elements for:
What is NRT control in qRT PCR?
A no reverse transcriptase control (NRT) or minus reverse transcriptase control (MRT) involves carrying out the reverse transcription step of a qRT-PCR experiment in the absence of reverse transcriptase. This control assesses the amount of DNA contamination present in an RNA preparation. 3.
What is RT 2 SYBR ® Green qPCR Mastermix?
The high-quality primer design and RT 2 SYBR ® Green qPCR Mastermix formulation enable the PCR array to amplify 96 or 384 different gene-specific products simultaneously under uniform cycling conditions.
Can RT-PCR be used to identify individual genes in mitochondria?
There are less than a dozen genes encoded by the mitochondrial genome (all other mitochondrial proteins are encoded by nuclear genes), and they are all transcribed as one transcript (just like any prokaryote), so distinguishing the expression of individual genes by real-time RT-PCR is not possible. What is the RT² Profiler PCR Array?