What includes in the biosafety level 2?
BSL-2 laboratories are used to study moderate-risk infectious agents or toxins that pose a moderate danger if accidentally inhaled, swallowed, or exposed to the skin. Design requirements for BSL-2 laboratories include hand washing sinks, eye washing stations, and doors that close and lock automatically.
What are the requirements for a BSL 2 lab?
In addition to BSL 1 expectation, the following practices are required in a BSL 2 lab setting:
- Appropriate personal protective equipment (PPE) must be worn, including lab coats and gloves.
- All procedures that can cause infection from aerosols or splashes are performed within a biological safety cabinet (BSC).
What is an example of a biosafety level 2 agent?
The agents require Biosafety Level 2 containment. Examples of BSL-2 organisms are: Mycobacterium, Streptococcus pneumonia, Salmonella choleraesuis. BSL-3 risk group contains biological agents that usually cause serious disease (human, animal or plant) or that can result in serious economic consequences.
What does a biosafety level 2 mean?
The biological material used in a BSL-2 laboratory consists of bacteria, viruses, and organisms associated with human diseases. The potential pathogenic or infectious organisms subject to BSL-2 standards pose a moderate hazard to healthy adult humans.
What biosafety cabinet can be installed in a BSL-2 laboratory?
For Biosafety Level 2 applications involving toxic chemicals or radionuclides, a Class II- B type cabinet must be installed. Class II-B cabinets do not allow in-room venting of exhaust air and are thus appropriate for such uses.
Which of the following material’s must be handled at BSL-2?
Gloves and Eye Protection Gloves must be worn while working at the BSL2 level.
What is the minimum PPE you should wear in the lab when working at BSL 2?
Wear appropriate PPE when working with infectious agents. Standard BSL-2 PPE consists of a dedicated lab coat, gloves, and eye protection. Other PPE may be required (see EH&S PPE Assessment Guide). Do not wear PPE in public areas.
What biosafety cabinet can be installed in a BSL 2 laboratory?
Which of the following material’s must be handled at BSL 2?
What biosafety risks do Level 2 organisms pose?
Biosafety level two would cover work with agents associated with human disease, in other words, pathogenic or infectious organisms posing a moderate hazard. Examples are the equine encephalitis viruses and HIV when performing routine diagnostic procedures or work with clinical specimens.
What is Class II A2 biosafety cabinet?
A Class II, Type A2 Biosafety Cabinet (BSC) provides personnel, product, and environmental protection through filtered air, laminar or unidirectional air, and a motor blower. Room air is drawn in through the front grill of the cabinet.
What are the basic principles of Biosafety Level 2?
Basics of Biosafety Level 2 Limit access to work areas. Close doors during work with research materials. Post biohazard warning signs at access points and on equipment containing or contaminated by potentially infectious materials.
What is bsl2 biosafety?
Biosafety Level 2 (BSL2) practices, equipment, and facility design are applicable to clinical, diagnostic, teaching, and other laboratories in which work is done with moderate-risk agents that are present in the community and associated with human.
How do you determine biosafety levels?
The primary risks that determine levels of containment are infectivity, severity of disease, transmissibility, and the nature of the work conducted. Origin of the microbe, or the agent in question, and the route of exposure are also important. The biosafety levels range from BSL-1 to BSL-4.
What is biosafety?
What is Biosafety? Biosafety is the application of safety precautions that reduce a laboratorian’s risk of exposure to a potentially infectious microbe and limit contamination of the work environment and, ultimately, the community. What are Biosafety Levels (BSLs)?